Cataloging the CCDI Childhood Cancer Data Catalog (CCDC)
Here at the Data Lab, we're all about, well, data! We believe that data sharing and accessibility is key to accelerating the research process, and ultimately to improving outcomes for childhood cancer patients. So, we were excited to learn that one of the goals of the NCI/NIH initiative, the Childhood Cancer Data Initiative (CCDI), is to build up a Data Ecosystem that will facilitate pediatric cancer researchers' ability to explore and collect data from disparate resources. Although this Ecosystem is still in the early stages, several components are already being developed and are available for researchers to use! One component that is particularly interesting to us is the CCDI's Childhood Cancer Data Catalog (CCDC). According to NCI,
The CCDI Childhood Cancer Data Catalog is an inventory of childhood cancer data repositories from across the childhood cancer research community. This catalog will make it easier for researchers, doctors, and citizen scientists to find data that will help them. Each resource page includes a summary description, data content types, and links to access the data. The inventory includes childhood cancer repositories, registries, data commons, websites, tools, and catalogs that manage and refer to data.
First, what is the CCDC? It's a submission-based service, meaning researchers can use a template form to submit their data resources as a way to promote resource awareness and usage within the pediatric cancer research community. Importantly, as the name implies, the CCDC is a catalog, not a database –- it does not host data directly, nor does it provide access to controlled data. Instead, the CCDC offers a centralized location for researchers to identify external data resources that may suit their needs which researchers can then further investigate on their own. Therefore, the CCDC's primary role is to lower the barrier to identifying potential resources of interest.
We were eager to learn more about the CCDC's potential, so we went ahead and "cataloged the data catalog" to develop a deeper sense of the landscape of existing data in pediatric cancer. What types of data are commonly available, and what types of analyses can they support? How do available data resources relate to one another? Can we at the Data Lab use any existing data in the catalog to improve our products, resources and/or training workshops, and similarly, could the CCDC be a suitable place to share our data? Answering these questions gives us insight into how we can best position ourselves to support cancer researchers in the age of data-intensive research, and here we'll pass some of what we found along to you! We'll note that there's a lot of ground here that we can't cover in this one blog post, but we hope this gives you a sense of what you can expect from the CCDC.
Within the CCDC, resources are generally partitioned into one of five categories (catalogs, knowledge bases, programs, registries, and repositories) depending on what kind of datasets they contain and how that data was originally collected. The CCDC offers a comprehensive glossary of terms and a PDF User Guide to help get you started exploring these resources, as well as release notes to help you keep tabs on how the catalog is being updated over time.
What types of data are commonly available, and what types of analyses can they support?
As of September 2022, the CCDC lists 100 datasets across 33 different resources which span a wide array of datatypes: "omics" datasets (both controlled-access and open summarized data), imaging, xenograft resources, cell lines, survivorship data, clinical trial results, and resources linking cancer genetic variants to clinically relevant outcomes. This breadth of data types means the CCDC caters broadly to all types of pediatric cancer researchers. Further, some of these resources are not limited to data but contain internal analytics and/or visualization tools that support data exploration or hypothesis generation. For example, St. Jude Cloud is a repository resource listed in the CCDC that both hosts genomic (much of which is controlled and must be requested) and clinical data as well as provides a robust cloud computing environment to interactively explore and analyze its hosted data. Another CCDC-listed registry resource, the National Childhood Cancer Registry Explorer (NCCR), offers data exports and options to make custom visualizations in the browser to explore incidence and survival statistics of pediatric and AYA (adolescent and young adult) cancer patients.
Importantly, not all resources are pediatric-specific, but all resources should contain at least some datasets of pediatric and/or AYA cancer, and this information is described on a given dataset and/or resource's summary page in the CCDC. These summary pages further provide additional metadata about dataset contents, often including number of cases and/or samples, case disease diagnosis, sample tissue location, case sex, case age at diagnoses, and any publications associated with the data. This information is generally collected during the submission process, so what kind of metadata is available for a given dataset will necessarily vary resource-to-resource. But, based on our experience, the CCDC maintainers can help you identify relevant metadata to include with your submission that is in line with what they would like to track.
How do available data resources relate to one another?
Something the CCDC highlights about the landscape of pediatric data is that there can be a lot of overlap among existing resources. While some datasets are unique to one resource, other datasets are embedded across several resources. One such example of this kind of overlap is data from the TARGET initiative, an NCI-sponsored cross-institutional program that genomically characterized several types of pediatric cancers. TARGET encompasses eleven sub-studies in a collaborative network of teams each studying different diseases and/or disease stages, such as Wilms Tumor and neuroblastoma. TARGET data includes open clinical metadata about patients and samples, open summarized genomic data as well as controlled raw genomics files whose access can be requested via dbGAP, an NIH-hosted repository for biological data. Although each TARGET subproject has its own dbGAP entry (for example, the Rhabdoid tumor sub-study and the Osteosarcoma sub-study), there is also an overall dbGAP page for the whole TARGET project where all requests for most of the controlled data should be directed.
Within the CCDC, individual TARGET sub-studies are recorded as "datasets," meaning there are eleven total datasets associated with TARGET. In this case, a given TARGET dataset comprehensively refers to all clinical/patient metadata, controlled genomic data, and summarized genomic data associated with a given TARGET sub-study.
As of September 2022, these eleven datasets are present in several of the 33 CCDC-listed resources:
- TARGET itself is a program resource in the CCDC that directs to the TARGET project homepage. This resource contains the eleven datasets - the TARGET sub-studies - and links are provided to the associated dbGAP Studies where controlled data can be requested.
- The Genomic Data Commons (GDC) is a repository resource in the CCDC. The GDC is an NCI-sponsored database of cancer genomic studies and hosts both open and controlled-access datasets. Within the CCDC, this resource lists eleven curated pediatric cancer datasets, one of which is TARGET. In fact, the GDC is the repository that contains controlled-access TARGET data - if one acquires access through dbGAP, one will be able to obtain the data from the GDC!
- Kids First (Gabriella Miller Kids First Pediatric Research Program) is a repository resource in the CCDC that offers both data export and analysis/visualization tools for pediatric cancer datasets. Within the CCDC, this resource lists fifteen different datasets, two of which are TARGET subprojects (TARGET AML and TARGET Neuroblastoma), and links for each are provided both to the GDC and to the relevant dbGAP study. These projects are listed because Kids First was a collaborator on these specific TARGET substudies.
- PedcBioPortal for Integrated Childhood Cancer Genomics is a repository resource within the CCDC that lists a single dataset: All data within the PedcBioPortal merged into a single overarching dataset for the purposes of reporting metadata (e.g. number of cases, diagnosis counts, case ages, etc.) within the CCDC. This CCDC entry provides a link to the PedcBioPortal home page, where users can find many distinct datasets which can be separately explored, visualized, and analyzed. Among these datasets are ten of the TARGET subprojects. The PedcBioPortal primarily provides an opportunity to interactively explore these data in the browser, but it also provides a link back to the TARGET Data Matrix for further information.
Based on our exploration of these TARGET-associated resources, we've summarized how you can interact with TARGET data in each one. (Figure 1)
How can the CCDC help us help you?
To explore how we at the Data Lab might use the CCDC to support pediatric cancer researchers, we did a little spelunking through the catalog itself! Since many of our initiatives focus on transcriptomic data, we compiled a list of RNA-seq and/or microarray datasets from pediatric tumor and/or xenografts in CCDC-listed resources. Next up, we're planning to dig through these datasets in a bit more depth to see how we might leverage these data. For example, we'll be looking for any openly available datasets that might be used as example datasets for trainees to analyze in our workshops.
While thinking about how CCDC data might support our resources, we got to thinking more about how we can work with the CCDC too! Currently, we're in the process of submitting the Single-Cell Pediatric Cancer Atlas (ScPCA), an open-access database of uniformly processed single-cell transcriptomic data from pediatric cancer clinical samples and xenografts, as a resource to the CCDC. You can read more about the ScPCA in this blog post. Our goal is for the ScPCA datasets to be part of the next quarterly CCDC release, so keep your eyes peeled!
In conclusion, we're excited to see this increased emphasis on data sharing in pediatric cancer research, and we hope you have a better sense now of how the CCDC might support your work as well! You can also support the CCDC's growth by submitting your datasets, and based on our experience, the CCDC maintainers will be glad to help you navigate the submission process. We look forward to following along as the catalog continues to develop.
On this blog, we share our expertise with the scientific community. You can expect to read technical content about our processes, information about our products and services, and much more. Subscribe here to receive updates!
Here at the Data Lab, we're all about, well, data! We believe that data sharing and accessibility is key to accelerating the research process, and ultimately to improving outcomes for childhood cancer patients. So, we were excited to learn that one of the goals of the NCI/NIH initiative, the Childhood Cancer Data Initiative (CCDI), is to build up a Data Ecosystem that will facilitate pediatric cancer researchers' ability to explore and collect data from disparate resources. Although this Ecosystem is still in the early stages, several components are already being developed and are available for researchers to use! One component that is particularly interesting to us is the CCDI's Childhood Cancer Data Catalog (CCDC). According to NCI,
The CCDI Childhood Cancer Data Catalog is an inventory of childhood cancer data repositories from across the childhood cancer research community. This catalog will make it easier for researchers, doctors, and citizen scientists to find data that will help them. Each resource page includes a summary description, data content types, and links to access the data. The inventory includes childhood cancer repositories, registries, data commons, websites, tools, and catalogs that manage and refer to data.
First, what is the CCDC? It's a submission-based service, meaning researchers can use a template form to submit their data resources as a way to promote resource awareness and usage within the pediatric cancer research community. Importantly, as the name implies, the CCDC is a catalog, not a database –- it does not host data directly, nor does it provide access to controlled data. Instead, the CCDC offers a centralized location for researchers to identify external data resources that may suit their needs which researchers can then further investigate on their own. Therefore, the CCDC's primary role is to lower the barrier to identifying potential resources of interest.
We were eager to learn more about the CCDC's potential, so we went ahead and "cataloged the data catalog" to develop a deeper sense of the landscape of existing data in pediatric cancer. What types of data are commonly available, and what types of analyses can they support? How do available data resources relate to one another? Can we at the Data Lab use any existing data in the catalog to improve our products, resources and/or training workshops, and similarly, could the CCDC be a suitable place to share our data? Answering these questions gives us insight into how we can best position ourselves to support cancer researchers in the age of data-intensive research, and here we'll pass some of what we found along to you! We'll note that there's a lot of ground here that we can't cover in this one blog post, but we hope this gives you a sense of what you can expect from the CCDC.
Within the CCDC, resources are generally partitioned into one of five categories (catalogs, knowledge bases, programs, registries, and repositories) depending on what kind of datasets they contain and how that data was originally collected. The CCDC offers a comprehensive glossary of terms and a PDF User Guide to help get you started exploring these resources, as well as release notes to help you keep tabs on how the catalog is being updated over time.
What types of data are commonly available, and what types of analyses can they support?
As of September 2022, the CCDC lists 100 datasets across 33 different resources which span a wide array of datatypes: "omics" datasets (both controlled-access and open summarized data), imaging, xenograft resources, cell lines, survivorship data, clinical trial results, and resources linking cancer genetic variants to clinically relevant outcomes. This breadth of data types means the CCDC caters broadly to all types of pediatric cancer researchers. Further, some of these resources are not limited to data but contain internal analytics and/or visualization tools that support data exploration or hypothesis generation. For example, St. Jude Cloud is a repository resource listed in the CCDC that both hosts genomic (much of which is controlled and must be requested) and clinical data as well as provides a robust cloud computing environment to interactively explore and analyze its hosted data. Another CCDC-listed registry resource, the National Childhood Cancer Registry Explorer (NCCR), offers data exports and options to make custom visualizations in the browser to explore incidence and survival statistics of pediatric and AYA (adolescent and young adult) cancer patients.
Importantly, not all resources are pediatric-specific, but all resources should contain at least some datasets of pediatric and/or AYA cancer, and this information is described on a given dataset and/or resource's summary page in the CCDC. These summary pages further provide additional metadata about dataset contents, often including number of cases and/or samples, case disease diagnosis, sample tissue location, case sex, case age at diagnoses, and any publications associated with the data. This information is generally collected during the submission process, so what kind of metadata is available for a given dataset will necessarily vary resource-to-resource. But, based on our experience, the CCDC maintainers can help you identify relevant metadata to include with your submission that is in line with what they would like to track.
How do available data resources relate to one another?
Something the CCDC highlights about the landscape of pediatric data is that there can be a lot of overlap among existing resources. While some datasets are unique to one resource, other datasets are embedded across several resources. One such example of this kind of overlap is data from the TARGET initiative, an NCI-sponsored cross-institutional program that genomically characterized several types of pediatric cancers. TARGET encompasses eleven sub-studies in a collaborative network of teams each studying different diseases and/or disease stages, such as Wilms Tumor and neuroblastoma. TARGET data includes open clinical metadata about patients and samples, open summarized genomic data as well as controlled raw genomics files whose access can be requested via dbGAP, an NIH-hosted repository for biological data. Although each TARGET subproject has its own dbGAP entry (for example, the Rhabdoid tumor sub-study and the Osteosarcoma sub-study), there is also an overall dbGAP page for the whole TARGET project where all requests for most of the controlled data should be directed.
Within the CCDC, individual TARGET sub-studies are recorded as "datasets," meaning there are eleven total datasets associated with TARGET. In this case, a given TARGET dataset comprehensively refers to all clinical/patient metadata, controlled genomic data, and summarized genomic data associated with a given TARGET sub-study.
As of September 2022, these eleven datasets are present in several of the 33 CCDC-listed resources:
- TARGET itself is a program resource in the CCDC that directs to the TARGET project homepage. This resource contains the eleven datasets - the TARGET sub-studies - and links are provided to the associated dbGAP Studies where controlled data can be requested.
- The Genomic Data Commons (GDC) is a repository resource in the CCDC. The GDC is an NCI-sponsored database of cancer genomic studies and hosts both open and controlled-access datasets. Within the CCDC, this resource lists eleven curated pediatric cancer datasets, one of which is TARGET. In fact, the GDC is the repository that contains controlled-access TARGET data - if one acquires access through dbGAP, one will be able to obtain the data from the GDC!
- Kids First (Gabriella Miller Kids First Pediatric Research Program) is a repository resource in the CCDC that offers both data export and analysis/visualization tools for pediatric cancer datasets. Within the CCDC, this resource lists fifteen different datasets, two of which are TARGET subprojects (TARGET AML and TARGET Neuroblastoma), and links for each are provided both to the GDC and to the relevant dbGAP study. These projects are listed because Kids First was a collaborator on these specific TARGET substudies.
- PedcBioPortal for Integrated Childhood Cancer Genomics is a repository resource within the CCDC that lists a single dataset: All data within the PedcBioPortal merged into a single overarching dataset for the purposes of reporting metadata (e.g. number of cases, diagnosis counts, case ages, etc.) within the CCDC. This CCDC entry provides a link to the PedcBioPortal home page, where users can find many distinct datasets which can be separately explored, visualized, and analyzed. Among these datasets are ten of the TARGET subprojects. The PedcBioPortal primarily provides an opportunity to interactively explore these data in the browser, but it also provides a link back to the TARGET Data Matrix for further information.
Based on our exploration of these TARGET-associated resources, we've summarized how you can interact with TARGET data in each one. (Figure 1)
How can the CCDC help us help you?
To explore how we at the Data Lab might use the CCDC to support pediatric cancer researchers, we did a little spelunking through the catalog itself! Since many of our initiatives focus on transcriptomic data, we compiled a list of RNA-seq and/or microarray datasets from pediatric tumor and/or xenografts in CCDC-listed resources. Next up, we're planning to dig through these datasets in a bit more depth to see how we might leverage these data. For example, we'll be looking for any openly available datasets that might be used as example datasets for trainees to analyze in our workshops.
While thinking about how CCDC data might support our resources, we got to thinking more about how we can work with the CCDC too! Currently, we're in the process of submitting the Single-Cell Pediatric Cancer Atlas (ScPCA), an open-access database of uniformly processed single-cell transcriptomic data from pediatric cancer clinical samples and xenografts, as a resource to the CCDC. You can read more about the ScPCA in this blog post. Our goal is for the ScPCA datasets to be part of the next quarterly CCDC release, so keep your eyes peeled!
In conclusion, we're excited to see this increased emphasis on data sharing in pediatric cancer research, and we hope you have a better sense now of how the CCDC might support your work as well! You can also support the CCDC's growth by submitting your datasets, and based on our experience, the CCDC maintainers will be glad to help you navigate the submission process. We look forward to following along as the catalog continues to develop.
On this blog, we share our expertise with the scientific community. You can expect to read technical content about our processes, information about our products and services, and much more. Subscribe here to receive updates!
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In our last blog post, we shared some of the tools and methods we are using in the Open Single-cell Pediatric Cancer Atlas (OpenScPCA) project to ensure that the analysis code remains usable and runnable throughout the project. That post mainly focused on some of the most dynamic phases of the project, when contributors are adding new analysis modules and updating existing ones with more refined results. Here, we will discuss the test data that enables the methods and our approach to running the full set of analyses on real data.
Applications are open for the Data Lab's next training workshop! We will cover advanced topics in the analysis of single-cell RNA-seq data for researchers studying pediatric cancer. The 3-day course will take place December 10-12, 2024 from 9am-5pm Eastern time in Bala Cynwyd, PA, just outside of Philadelphia.
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Applications are open for the Data Lab's next workshop! We will hold a Reproducible Research Practices Course on October 23-24, 2024 in Milwaukee, WI. Instructors will introduce principles and techniques to achieve reproducible results in computational cancer research. We’ll show you the fundamentals of commonly used approaches in reproducibility that you can apply to increase the impact of your research by making your findings more robust and reliable! To ensure that workshop attendees have a great hands-on experience, a very limited number of seats will be available.
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So you recently did some single-cell RNA sequencing and are working on analyzing your data. You’ve already quantified the gene expression data, performed any filtering, and normalized your data, but now what? You know you want to perform differential expression analysis or that you need to annotate the cell types found in your data, but there are so many different tools and methods for performing these analyses. How do you know which one is the best method for your dataset? Don’t worry, we’ve all been there – even experts in the single-cell field have been there.
The Open Single-cell Pediatric Cancer Atlas (OpenScPCA) is an open, collaborative project to analyze data from the Single-cell Pediatric Cancer Atlas (ScPCA) Portal, which currently holds over 500 samples from over 50 pediatric cancer types. OpenScPCA uses an open contribution model designed to allow experts worldwide to contribute and rapidly share the results of analyses in real time. The project was officially launched in April 2024.
In March 2022, we launched the Single-cell Pediatric Cancer Atlas (ScPCA) Portal to make uniformly processed single-cell and single-nuclei RNA-Seq data widely available to the childhood cancer research community. Initially, all data available on the Portal was generated through grants funded by Alex’s Lemonade Stand Foundation (ALSF) as part of the ScPCA project. But enabling access to ALSF-funded data was just the beginning of our vision.Sharing is key to ensuring the Portal’s continued growth. Our sights were set on allowing more pediatric cancer researchers to contribute data to the ScPCA Portal.
The Data Lab has just launched the brand new Open Single-cell Pediatric Cancer Atlas (OpenScPCA) project! This open, collaborative project aims to analyze data from the ScPCA Portal, which currently holds 500 samples from over 50 pediatric cancer types. We are seeking contributors with diverse skills and expertise to join the project!
We are excited to announce that our next virtual workshop, Introduction to Single-cell RNA-Seq, will run from June 10-14, 2024! In this workshop, Data Lab staff will introduce researchers studying pediatric cancer to the R programming language, the Tidyverse R packages for data science, single-cell RNA-seq data analysis, and annotating cell types.
Applications are open for the Data Lab's next workshop! We are holding a two-day course on Reproducible Research Practices and the Open Single-cell Pediatric Cancer Atlas (OpenScPCA) project from May 14-15, 2024. Please note that the OpenScPCA module is an optional part of the workshop. The course begins with an introduction to principles and techniques to achieve reproducible results in computational cancer research. On day two, you can choose to continue the workshop and learn how to put your skills to use for OpenScPCA, our new pediatric cancer research project.
Are you attending the American Association for Cancer Research (AACR) annual meeting in San Diego, CA? Visit the Alex’s Lemonade Stand Foundation (ALSF) Grants and Data Lab teams at booth 3755 in the exhibit hall from April 7-10 and during poster sessions on April 8. We will announce a new collaborative project and share exciting news about the Single-cell Pediatric Cancer Atlas Portal and training opportunities!
Did you know that 70% of the Alex’s Lemonade Stand Foundation (ALSF) Childhood Cancer Data Lab team are currently women? Advancing our mission to empower childhood cancer researchers with knowledge, data, and tools would not be possible without their expertise. On the International Day of Women and Girls in Science, we are excited to introduce you to these women who integrate science, engineering, and design to tackle some of the greatest challenges faced by the pediatric cancer research community!
I have a confession to make: I am lazy. Ok, maybe that's too strong. Let's go for a euphemism instead: I am efficient. I love learning handy tricks that make my life easier and make my job smoother with fewer hiccups along the way. This is one part of why, here in the Data Lab, we love automation - why waste our time on rote, repetitive, housekeeping tasks when we can get the bots to do it for us? In this blog post, we'll highlight a few tips about how you can use RStudio to code more efficiently.
Writing source code is a significant part of data-intensive biomedical research. Everything from cleaning and pre-processing data to generating publication figures can be accomplished programmatically. Increasingly, funding agencies and journals require researchers to share their code. To pick a few examples, the Data Lab’s parent organization, Alex’s Lemonade Stand Foundation (ALSF), has such a requirement for awardees, and PLoS Computational Biology requires authors to make code underlying results and conclusions available.
There is an old joke in computer science about how there are only two hard things: cache invalidation, naming things, and off-by-one errors. I’ll leave aside the first one as beyond my own expertise, but the second comes up all the time in my work as a biological data scientist. Naming variables and functions in my code is a constant struggle, but one I have to deal with on my own or with my team. Much bigger problems come up when trying to deal with all the various ways that people across the world use names when talking about the diseases they work on, the types of cells they are looking at, the experimental methods they are using, and just about every other aspect of their studies.
Applications are open for the Data Lab's next workshop! We will be holding a Reproducible Research Practices Course in-person on October 24-25, 2023. Instructors will introduce principles and techniques to achieve reproducible results in computational cancer research. We’ll show you the fundamentals of commonly-used approaches in reproducibility that you can apply to increase the impact of your research by making your findings more robust and reliable! To ensure that workshop attendees have a great hands-on experience, there will be a very limited number of seats available.
At the Center for Data-Driven Discovery in Biomedicine (D3b), I lead the Bioinformatics Translational Pediatric Oncology Team, a team of bioinformatics scientists. Our mission is to advance pediatric oncology research and precision medicine through collaboration and development of open-source analytical tools, frameworks, and data resources. In 1998, I lost my four year old cousin John Matthew to a brain tumor we now know was likely a diffuse intrinsic pontine glioma. So, it was bittersweet for me to see the Open Pediatric Brain Tumor Atlas (OpenPBTA) manuscript published in Cell Genomics on the last day of brain tumor awareness month this past year. But let’s rewind.
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In 2019, Alex’s Lemonade Stand Foundation (ALSF) established the Single-cell Pediatric Cancer Atlas (ScPCA) through awards for data generation and to create an atlas of single-cell gene expression profiles of pediatric cancers of different types and from different organ sites. The Data Lab launched the ScPCA Portal in 2022 to make uniformly processed, summarized single-cell and single-nuclei RNA-seq data and de-identified metadata available for download. The ScPCA Portal also supports other data modalities, such as bulk RNA-seq, CITE-seq, and spatial transcriptomics. The ScPCA Portal currently hosts data for over 500 pediatric tumor and patient-derived xenograft samples from more than 50 cancer types, and continues to grow. The Data Lab is seeking contributions to the ScPCA Portal from researchers with existing single-cell datasets.
We are excited to announce that our next workshop, Introduction to Single-cell RNA-Seq, will take place in-person from June 13-15, 2023! Data Lab staff will introduce researchers studying pediatric cancer to the R programming language, the Tidyverse R packages for data science, single-cell RNA-seq data analysis, annotating cell types, and more. The 3-day course will take place from 9am-5pm Eastern time in Bala Cynwyd, PA, just outside of Philadelphia. Travel reimbursement (up to a certain amount) is available for qualifying participants.
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Are you attending the American Association for Cancer Research (AACR) annual meeting in Orlando, FL this year? Visit Alex's Lemonade Stand Foundation (ALSF) at booth 369 in the exhibit hall from April 16-19! You'll find information about ALSF's grants program, the Childhood Cancer Data Lab and more. The Data Lab will also be holding office hours during select time slots.
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In September 2022, the Open Pediatric Brain Tumor Atlas (OpenPBTA) project culminated (for now) in a preprint on bioRxiv. This project, started in late 2019 and co-organized with the Center for Data Driven Discovery in Biomedicine (D3b) at Children’s Hospital of Philadelphia (CHOP), is a collaborative effort to comprehensively describe the Pediatric Brain Tumor Atlas (PBTA), a collection of multiple data types from tens of tumor types (read more about why crowdsourcing expertise for the study of pediatric brain tumors is important here). The project is designed to allow for contributions from experts across multiple institutions. We’ve conducted analysis and drafting of the manuscript openly on the version-control platform GitHub from the project’s inception to facilitate those contributions.
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The Data Lab is excited to announce that our next training workshop will be held in-person from January 31-February 2, 2023! During this workshop, we will cover advanced topics in the analysis of single-cell RNA-seq data for researchers studying pediatric cancer. The 3-day course will take place from 9am-5pm Eastern time in Bala Cynwyd, PA, just outside of Philadelphia. Travel reimbursement is available for qualifying participants.
Welcome to the Data Lab’s December Scientific Community Bulletin! Each month we share upcoming opportunities from Alex’s Lemonade Stand Foundation (ALSF), the Data Lab, and other events that we have gathered from a variety of science and research organizations. Subscribe to our blog to be alerted about future Scientific Community Bulletin posts!
In this blog post, I’d like to give an overview of the refine.bio refactoring process and web accessibility considerations. Through this process, our goal is to enhance the site usability and performance by improving the code quality and making the application more accessible. But before going into more details about them, let me provide you a quick history of refine.bio.
Welcome to the Data Lab’s November Scientific Community Bulletin! Each month we share upcoming opportunities from Alex’s Lemonade Stand Foundation (ALSF), the Data Lab, and other events that we have gathered from a variety of science and research organizations. Subscribe to our blog to be alerted about future Scientific Community Bulletin posts!
Welcome to the October Scientific Community Bulletin! Each month we share upcoming opportunities from Alex’s Lemonade Stand Foundation (ALSF), the Data Lab, and other events that we have gathered from a variety of science and research organizations. Subscribe to our blog to be alerted about future Scientific Community Bulletin posts!
Welcome to the September Scientific Community Bulletin! Each month we share upcoming opportunities from Alex’s Lemonade Stand Foundation (ALSF), the Data Lab, and other events that we have gathered from a variety of science and research organizations. Subscribe to our blog to be alerted about future Scientific Community Bulletin posts!
At the Data Lab, we are constantly looking for ways to enhance the tools we build for pediatric cancer researchers. Earlier this year, we launched the Single-cell Pediatric Cancer Atlas portal, a database of uniformly-processed single-cell data from pediatric cancer clinical samples. One way we felt the portal could be even more beneficial to pediatric cancer researchers is with a ready-to-go workflow that takes in single-cell data and prepares it for downstream analyses such as unsupervised clustering.
The Data Lab is excited to announce our next virtual workshop running from September 19-23, 2022! In this workshop, Data Lab staff will introduce researchers studying pediatric cancer to the R programming language, the Tidyverse R packages for data science, single-cell RNA-seq data analysis, and pathway analysis.
The Data Lab teaches data science courses targeted toward pediatric cancer researchers that introduce topics such as analysis of gene expression in bulk and single-cell data and principles of reproducible research. I wrote previously about how we use RStudio Server for our remote courses to simplify setup, and I wanted to write a bit more about some of the instructional practices we use so that our participants get the best experience we can provide. In particular, I wanted to talk about our use of live coding to facilitate active learning, and one of the tools we developed to make our course development just a bit easier.
Welcome to the August Scientific Community Bulletin! Each month we share upcoming opportunities from Alex’s Lemonade Stand Foundation (ALSF), the Data Lab, and other events that we have gathered from a variety of science and research organizations.
Often when building a server-client web application, we will encounter a situation where we want to send requests to our API in the chronological order that they occur on the client. Due to the asynchronous nature of these requests, it might not be possible to send them in the same callback for the event that triggered them. This is because we want to use the response from the previous request to craft our current one. A solution to this problem would be to implement a queue. Instead of calling the API immediately after events occur, implementing a queue ensures the latest data is sent with any request.
Welcome to the July Scientific Community Bulletin! Each month we share upcoming opportunities from Alex’s Lemonade Stand Foundation (ALSF), the Data Lab, and other events that we have gathered from a variety of science and research organizations. Subscribe to our blog to be alerted about future Scientific Community Bulletin posts!
Welcome to the Childhood Cancer Data Lab’s new blog feature, the monthly Scientific Community Bulletin! At the start of each month, we will share upcoming opportunities from Alex’s Lemonade Stand Foundation (ALSF), the Data Lab, and other events that we have gathered from a variety of science and research organizations. Our goal is to promote learning opportunities and highlight some of the excellent resources that our community provides.
At the Data Lab, our science team has a practice where an individual team member shares something that they recently figured out (or didn’t totally figure out yet) on a biweekly basis. We call this short 5-10 minute presentation How I Solved This, and it’s a great way to formally share (often hard-won) knowledge with each other. In this post, we thought we’d share how we solved something with the `renv` package with you.
The Childhood Cancer Data Lab builds resources guided by the most pressing needs of our primary users: pediatric cancer researchers. As the Data Lab's UX Designer, I conduct research activities with scientists like usability evaluations, semi-structured interviews, and card sorts to gain insight into their activities, processes, pain-points, and behaviors. I work with scientists and engineers at the Data Lab to use this information to improve existing products and services or to create new ones.
The Data Lab is excited to announce that our next training workshop is taking place in-person on Friday, June 10, 2022! During this full day workshop, instructors will introduce principles and techniques to achieve reproducible results in computational cancer research. We’ll show you the fundamentals of commonly-used approaches in reproducibility that you can apply to increase the impact of your research by making your findings more robust and reliable!
The Childhood Cancer Data Lab is growing as a resource for pediatric cancer researchers and we have more to offer to our community now, than ever before. Transitioning to our new and improved website is an exciting milestone, and here, we look forward to sharing progress, introducing new initiatives, and cultivating more opportunities to support childhood cancer research. Welcome to our new virtual home!
The Single-cell Pediatric Cancer Atlas (ScPCA) project began in 2019 when Alex’s Lemonade Stand Foundation (ALSF) funded 10 awards for single-cell profiling of pediatric cancer samples. The goal was to produce an atlas of gene expression profiles for a variety of childhood cancer types from different organ sites.
The CCDL team includes science, engineering, and design expertise. Combining these three disciplines in different ways across projects enables us to carry out our mission.
Here at the CCDL we value putting publicly available data to work. For example, we are currently processing and normalizing 1.5 million publicly available gene expression samples totaling ~$1.5 billion research dollars expended.
Like many teams that work with large amounts of external software, we run into issues with our transitive dependencies. In general, transitive dependencies are a hard problem to solve.
Though technology can introduce great benefit into our lives, it is often accompanied by a substantial amount of time and some expected frustration before we can reap the rewards. The time spent learning a new technology is what we usually call a learning curve.
The workshop will last from 9AM to 5PM on October 14th, 15th, and 16th at the CCDL offices at 1429 Walnut St Philadelphia, PA, 19102.
MultiPLIER is a machine learning approach that brings big data to bear on rare diseases. It’s also an example of the scientific approach and ethos of the CCDL, and the publication is a great opportunity to share how the CCDL is developing new technologies to accelerate research into cures for childhood cancers!
The Childhood Cancer Data Lab powered by Alex's Lemonade Stand Foundation is hosting a workshop to introduce childhood cancer researchers to reproducible analysis of bulk and single-cell transcriptomic data.
The Childhood Cancer Data Lab (CCDL), an initiative of Alex's Lemonade Stand Foundation develops tools, trainings, and methods to empower childhood cancer researchers. The work at the CCDL is focused and impactful. There are multiple opportunities and challenges for you to apply and grow your skills as a scientist or as an engineer.
The Childhood Cancer Data Lab powered by Alex's Lemonade Stand Foundation is hosting a workshop to introduce childhood cancer researchers to reproducible analysis of bulk and single-cell transcriptomic data.
At this hands-on, 3-day session held in Houston, researchers learned data science skills that could accelerate their own work. Drawing on skills learned at the workshop, childhood cancer researchers can perform basic analyses of their work to make informed decisions on how to proceed with their own research. Don’t just take our word for it, though. Read more about the workshop’s incredibly valuable benefits through its attendees’ perspectives.
The goal of our refine.bio project is to download, process, and make available gene expression datasets that can be analyzed together, or in parts, depending on a researcher’s need. Childhood cancer researchers need to be able to use data generated through multiple profiling technologies including microarrays and RNA-sequencing.
There are countless log blog posts out there about the benefits of good logging, how to log well, and how much to log. Going through them all can be a real log blog slog. Wouldn't it be cool if you could log like this:logger.info("Something happened!", job=job.id, user=user.id) and get an easily searchable output.
Caffeine is a stimulant that can induce alertness in certain individuals when consumed at an appropriate quantity. Caffeine is often obtained by ingesting caffeine-containing solutions. However, no protocol for obtaining caffeine from dehydrated, roasted beans using materials typically available in a Philadelphia office has been described in the published literature.
Alex’s Lemonade Stand Foundation (ALSF) staunchly believes that stronger scientific sharing practices will accelerate the pace of discovery and finding cures for children with cancer. Robust sharing improves reproducibility, minimizes redundant studies and maximizes our return on research investment.
Earlier this year, Alex’s Lemonade Stand Foundation identified single-cell gene expression profiling as an opportunity to build an atlas of cell types within tumors that could be broadly reused by pediatric cancer researchers.
This year was a big one for the CCDL. In our mission to empower pediatric cancer experts poised for big discoveries with the knowledge, data and methods to reach them we launched a software product, developed and delivered training workshops on single-cell and bulk RNA-seq analysis, and hired our data science team among other milestones.
I’m a scientist at Sage Bionetworks, a nonprofit research organization in Seattle, WA. My work focuses on a family of rare pediatric diseases (NF): neurofibromatosis type 1, type 2, and schwannomatosis.
Our particular process is designed to source opportunities from our team members and external stakeholders, convert those opportunities into a set of potential goals, and then select the goals that we expect will most advance our mission.
The ability to restore scroll position is often critical for website usability. It helps users keep the flow of navigation when going back and forth between different pages. Most modern browsers take care of restoring the scroll position automatically, but it doesn’t always work for Single Page Applications where the content is generated on the client’s side, often asynchronously.
Carnegie Mellon University Libraries is partnering with the Childhood Cancer Data Lab (CCDL), founded by Alex’s Lemonade Stand Foundation, to host a Data Analysis workshop using CCDL materials.
The CCDL will have a team of scientists at the American Association for Cancer Research 2020 Annual Meeting in sunny San Diego! Our team members are excited to talk to researchers studying pediatric cancer at Booth 1601.
To help keep pediatric cancer research moving forward, here are 3 ways the CCDL is helping the research community during this time: refine.bio, virtual workshops, and the Open Pediatric Brain Tumor Atlas project.
We know that pandemic-related university closures mean that the demand for opportunities for pediatric cancer researchers to increase their analytical skills has never been higher. As such, we are delighted to announce a pilot virtual workshop running from May 4-8, 2020!
Here at the Childhood Cancer Data Lab, we value transparency and the practice of open science. Much of the work we’ve done and the products that we build hinge on the generosity and openness of other scientists. In this post, as part of National Brain Tumor Awareness month, we want to talk about a project that our science team has been working on over the last few months (and to do so in a way that aligns with our values).
The workshop will take place on June 22 - 26, 2020 from noon - 5pm Eastern. Each day consists of lectures and designated time for attendees to work on exercise materials and their own projects with CCDL staff available for consultation.
When the CCDL (along with everyone else) realized that we would have to conduct our bioinformatics training workshops remotely, we had to make some quick decisions about how we were going to do it. Most of the instructional materials for our in person workshops were already online, so we knew we had a good base to work from. We just needed to figure how to adapt the live instruction.
At Alex’s Lemonade Stand Foundation’s Childhood Cancer Data Lab, we’re excited to be helping out with an upcoming event hosted by the Children’s Tumor Foundation. If you participate, you may meet members of our team who are mentoring and judging.
The workshop will take place on March 22 - 26, 2021 from noon - 5pm Eastern. Each day consists of lectures and designated time for attendees to work on exercise materials and their own projects with CCDL staff available for consultation
The workshop will take place on June 28- July 2, 2021 from noon to 5pm eastern. Each day consists of lectures and designated time for attendees to work on exercise materials and their own projects with CCDL staff available for consultation.
Hack4Rare is a virtual event that calls for healthcare startups, developers, solutions architects, and hackathon enthusiasts to join researchers, clinicians and patients in developing solutions built around a number of rare diseases including neurofibromatosis, PTEN Hamartoma Tumor Syndrome, RASopathies and Desmoid Tumors.
The workshop will take place on September 20 - 24, 2021 from noon - 5pm Eastern. Each day consists of lectures and designated time for attendees to work on exercise materials and their own projects with CCDL staff available for consultation.
Introducing refine.bio examples. Here, users can access a variety of example analyses implemented in R, such as clustering and heat maps, differential expression analysis, and pathway analysis, for use with refine.bio data.
The workshop will take place on November 1-5, 2021 from noon to 5pm eastern. Each day consists of lectures and designated time for attendees to work on exercise materials and their own projects with our staff available for consultation.
I work at the Childhood Cancer Data Lab, where we use very big data to find cures for childhood cancers. To move data around the internet at very high speeds, we are forced to use a proprietary software suite called Aspera. If somebody could make a Free Software alternative, the future of the internet would be way more awesome! Best of all, you can be the one to do it!
When my daughter Alex was diagnosed with cancer and throughout her battle, we saw how our community of people rallied around our family. No one knew quite how to help, but they were willing to do whatever was needed to ease the burden we faced.
'Work smarter not harder’ is useless advice if you don’t know how to ‘work smarter’. But the Childhood Cancer Data Lab's work and processes may be the smartest I’ve ever had the pleasure of learning and adopting.
The Data Lab will hold our first virtual workshop of the year from March 14-18, 2022!In this workshop, we will introduce researchers studying pediatric cancer to the R programming language, the Tidyverse R packages for data science, single-cell RNA-seq data analysis, and pathway analyses.
At the Data Lab, we are big proponents of automating the boring stuff so we can spend more time thinking about the fun stuff. But how exactly do we do that, and what does it mean to automate the boring stuff?
November marked the final Childhood Cancer Data Lab training workshop for 2021. We held four week-long virtual workshops this year, teaching 88 researchers the data science skills they need to examine their own data.
Before working as a Data Scientist at the Childhood Cancer Data Lab, I spent time in my PhD and post-doctoral fellowship in two very different research environments. Each had their own unique way of doing research. I found that some things worked really well and others were not as successful.
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